The Effect of Fermentation Time on The Quality of Mocaf (Modified Cassava Flour) with Raw Material Bokor Genotype Cassava

Authors

DOI:

https://doi.org/10.23960/jtep-l.v13i1.12-26

Abstract

Cassava genotype Bokor has a fairly high content of beta carotene, which is beneficial for health and can also support the development of the functional food industry. MOCAF (Modified Cassava Flour) is a product made from cassava flour which is processed by modifying cassava cells through fermentation. This study aims to analyze the effect of long fermentation time on the characteristics of MOCAF from beta-carotene-rich cassava genotype Bokor. In this study, three variations of fermentation time were used, namely 15 hours, 24 hours and 40 hours. The research results showed that the time of fermentation affected the physicochemical properties of MOCAF. NIR analysis showed that the fermentation time affected the chemical composition of MOCAF. At 40 hours (last time of fermentation), there was a decrease in fat content with a value of 1.59% and an increase in water, protein and fibre content with a value of 13.71%, 3.94% and 2.28%, respectively. Proximate analysis showed that there was an effect of the length of fermentation time on the MOCAF content, namely at 40 hours, there was an increase in water content with a value of 10.10%, a decrease in dry matter, ash, protein, fat, fibre with a value of 89.90%, 0.60%, 0.78% respectively, 0.45%, and 1.29%. Scanning Electron Microscope (SEM) analysis shows the effect of fermentation time on the morphological properties of MOCAF starch granules. The longer the fermentation time, the more perforated or damaged the surface of the starch granules.

 

Keywords:  Fermentation time, MOCAF flour, NIR (Near Infrared), Proximate, SEM (Scanning Electron Microscope) 

Author Biographies

  • Erfa Kurnia Prastiwi, Muhammadiyah University of Surakarta
    Student Bachelor Degree, Program Study of Chemical Engineering, Faculty of Engineering
  • Rois Fatoni, Muhammadiyah University of Surakarta
    Dean Faculty of Engineering
  • Ahmad Fathoni, National Research and Innovation Agency (BRIN)
    Head of Research Center for Applied Microbiology
  • R. Haryo Bimo Setiarto, National Research and Innovation Agency (BRIN)
    Senior Researcher Food Microbiology in Research Center for Applied Microbiology
  • Ema Damayanti, National Research and Innovation Agency (BRIN)
    Senior Researcher Food Microbiology in Research Center for Food Process Technology

References

Aini, N. et al. (2009). Ubi kayu substitusi berbagai Tepung-tepungan, Forum Pascasarjana, 32(1), 33–34.

Aisah, A., Harini, N., & Damat, D. (2021). Pengaruh waktu dan suhu pengeringan menggunakan pengering kabinet dalam pembuatan MOCAF (modified cassava flour) dengan fermentasi ragi tape. Food Technology and Halal Science Journal, 4(2), 172–91. https://doi.org/10.22219/fths.v4i2.16595

Anindita, B.P., Antari, A.T., & Gunawan, S. (2019). Pembuatan MOCAF ( modified cassava flour ) dengan kapasitas 91000 ton/tahun. Jurnal Teknik ITS, 8(2), 170–175. https://doi.org/10.12962/j23373539.v8i2.45058

Asmoro, N.W. (2021). Characteristics and properties of modified cassava flour ( Mocaf ) and its benefits in food poducts. Journal of Food and Agricultural Product, 1(1), 34–43. https://doi.org/10.32585/jfap.v1i1.1755

Badriani, B., Fadillah, R., & Sukainah, A. (2020). The effect of mocaf flour substitution in the making of kasippi as an efforts to improve the quality of mandar typical traditional food. Jurnal Pendidikan Teknologi Pertanian, 6(2), 187–199.

Collins, T.J. (2007). ImageJ for microscopy. BioTechniques, 43(1), 25–30. https://doi.org/10.2144/000112517

Dewi, K.L., Aulina, D.E., Wulandari, F., & Maharani, S. (2002). Modifikasi pati dengan fermentasi (S. Cerevisiae) pada tepung pisang, tepung ubi ungu, dan tepung ketan hitam. Jurnal Universitas Pendidikan Indonesia, 7(2), 167–183. https://doi.org/10.17509/edufortech.v7i2.51624

Diniyah, N., Subagio, A., Sari, R.N.L., Yuwana, N. (2018). Sifat fisikokimia dan fungsional pati dari mocaf (modified cassava flour) varietas kaspro dan cimanggu. Jurnal Penelitian Pascapanen Pertanian, 15(2), 80-90.

Diniyah, N., Yuwana, N., Purnomo, B.H., & Subagio, A. (2018). Karakterisasi sera mocaf (modified cassava flour) dari ubi kayu varietas manis dan pahit. Jurnal Penelitian Pascapanen Pertanian, 15(3), 13-139.

Dipowaseso, D.A., Nurwanto, N., & Hintono, A.H. (2018). Karakteristik fisik dan daya oles selai kolang-kaling yang dibuat melalui subsitusi pektin dengan modified cassava flour (MOCAF) Sebagai Bahan Pengental. Jurnal Teknologi Pangan, 2(1), 1–7.

Fardiaz, S. (1992). Mikrobiologi Pangan. Jakarta. PT. Gramedia Pustaka Utama.

Ginting, S.P. & Krisnan, R. (2006). Pengaruh fermentasi menggunakan beberapa strain trichoderma dan masa inkubasi berbeda terhadap komposisi kimiawi bungkil inti sawit. In Seminar Nasional Teknologi Peternakan dan Veteriner, 939–944.

Herman, H., Rusli, R., Ilimu, E., H., Hamid, R., & Haeruddin, H. (2011). Analisis kadar mineral dalam abu buah nipa (nypa fructicans) Kaliwanggu Teluk Kendari Sulawesi Tenggara. Journal Of Tropical Pharmacy And Chemistry, 1(2), 107–113. https://doi.org/10.25026/jtpc.v1i2.17

Hersoelistyorini, W., Dewi, S.S., & Kumoro, A.C. (2015). Sifat fisikokimia dan organoleptik tepung mocaf (modified cassava flour) dengan fermentasi menggunakan ekstrak kubis. The 2nd University Research Coloquium, 10–17.

Hidayat, B. Kalsum, N., & Surfiana. (2009). Karakterisasi tepung ubi kayu modifikasi yang diproses menggunakan metode pragelatinisasi parsial. Jurnal Teknologi Industri, 14(2).

Karlinasari, L., Sabed, M., Wistara, N.J., Purwanto, A., & Wijayanto, H. (2012). Karakter spektra absorbansi NIR (near infrared) spektroskopi kayu acacia mangium WILLD pada 3 umur berbeda. Jurnal Ilmu Kehutanan, 6(1), 45–52. https://doi.org/10.22146/jik.3310

Kasmiran, A. (2011). Pengaruh lama fermentasi jerami padi dengan mikroorganisme lokal terhadap kandungan bahan kering, bahan organik, dan abu. Lentera, 11(1), 48–52.

Khasanah, Y., Nuryati, R., Mustikasari, A., & Astuti, I.W. (2021). The physicochemical and microbiological characteristics of fermented modified cassava flour (Mocaf) by using the dry starter. Jurnal Reset Teknologi Industri, 15(2), 168–178. https://doi.org/10.26578/jrti.v15i2.6674

Kuncari, E.S., Praptiwi., & Iskandarsyah. (2014). Evaluation, physical stability test and syneresis of gel containing minoxidil, apigenin and celery (apium graveolens L.) juice. Buleti Penelitian Kesehatan, 42(4), 213–222.

Kurniawan, S. (2010). Pengaruh lama fermentasi dan konsentrasi Ca(OH)2 untuk perendaman terhadap karakteristik tepung mocaf (modified cassava flour) varietas singkong pahit. Pandemir, 2.

Kustomo (2020). Uji karakterisasi dan mapping magnetit nanopartikel terlapisi asam humat dengan scanning electron microscope – energy dispersive X-Ray (SEM-EDX). Indonesian Journal of Chemical Science, 9(3), 149–153.

Laili, C.L., Suryani, C.L., & Setyowati, A. (2018). Inovasi Pangan Lokal Untuk Mendukung Ketahanan Pangan. Yogyakarta. Universitas Mercu Buana Yogyakarta.

Laksin, D.L. & Hubert, A.L. (1973). Handbook of food technology. Wesport: The AVI Publishing Co. Inc.

Lengkey, L.C., budiastra, I.W., & Kudang, B.(2013). Prediction model of moisture, fat, and free fatty acid content of physic nut (jatropha curcas l.) seed using near infrared (NIR) spectroscopy and partial east quare (PLS) method. Jurnal Littri, 19(4), 203–2011.

Mirzah & Muis, H. (2015). Improving nutrient quality of cassava peel waste by fermentation using the bacillus amyloliquefaciens. Jurnal Peternakan Indonesia, 17(2). https://doi.org/10.25077/jpi.17.2.131-142.2015

Muza’ki, K.A., Warsidah., & Nurdiansyah, Sy.I. (2022). Analisis kandungan proksimat kerang ale-ale (Meretrix Sp.) segar dan fermentasi. Jurnal Kimia Khatulistiwa, 10(1), 26–34.

Nur’utami, D.A., Fitrilia, T., & Oktavia, D. (2020). The effect of ermentation time to sensory and dough development properties in mocaf (modified cassava flour) bread. Jurnal Agroindustri Halal, 6(2), 197–204. https://doi.org/10.30997/jah.v6i2.3255

O’Brien, N.A., Hulse, C.A., Friedrich, D.M., Millihen, F.J.V, Gunten, M.K.V., Pfeifer, F., & Siesler, H.W. (2012). Miniature near-infrared (NIR) spectrometer engine for handheld applications. Next-Generation Spectroscopic Technologies V, 8374, 31-38. https://doi.org/10.1117/12.917983

Puspadewi, R., Adirestuti, P., & Anggreani, G. (2011). Aktivitas Metabolit Bakteri Lactobacillus plantarum dan Perannya dalam Menjaga Kesehatan Saluran Pencernaan. Konferensi Nasional Sains.

Putri, N.A. Herlina., & Subagio, A. (2018). Karakteristik mocaf (modified cassava flour) berdasarkan metode penggilingan dan lama Fermentasi. Jurnal Agroteknologi, 12(01), 79. https://doi.org/10.19184/j-agt.v12i1.8252

Rahayu, K. (1990). Teknologi Enzim. Yogyakarta: Penerbit Pusat Antar Universitas Pangan dan Gizi UGM.

Regina, O., Subrajad, H., & Syaflita, D. (2018). Measurement of Viscosity Uses an Alternative Viscometer Pengukuran Viskositas Menggunakan Viskometer Alternatif. Jurnal Geliga Sains, 6(2), 127–132. https://doi.org/10.31258/jgs.6.2.127-132

Sari, N.D., Setiyatwan, H., & Abun. (2016). The effect of long fermentation by bacillus licheniformis continued by saccharomyces cereviseae on shrimp waste on content of protein and glucose product, Jurnal Unpad, 5(4), 1-11.

Seveline., Heldyana, R., Kurniawati, S., (2020). The use of three species of lactic acid bacteria in the mocaf (modified cassava flour) production. Industria: Jurnal Teknologi dan Manajemen Agroindustri, 9(3), 163–172. https://doi.org/10.21776/ub.industria.2020.009.03.1

Subagio. (2006) .Ubi kayu substitusi berbagai tepung-tepungan. Food Review, 1(3), 18–22.

Sudarmonowati, E., Hartati, S., Kurniawati, S, Fathoni, A., & Harmoko, R. (2020). Pangan Fungsional Berbasis Ubi Kayu Kaya Beta Karoten, Pangan Fungsional Berbasis Ubi Kayu Kaya Beta Karoten. Jakarta. LIPI Press.

Suhery, W.N. Halim, A., & Lucida, H. (2013). Uji sifat fisikokimia mocaf (modified cassava flour) dan pati singkong termodifikasi untuk formulasi tablet. Jurnal Farmasi Indonesia, 6(3), 129–137.

Sulistiadi, S., Apriliani., & Kurniawan, A. (2021). Rancang desain alat pengayak modified cassava flour (mocaf) berdasarkan analisis kebutuhan, morfologi dan teknik. Journal of Agricultural Engineering, 10(1), 73. https://doi.org/10.23960/jtep-l.v10i1.73-84

Tandrianto, J. & Mintoko, D.K. (2014). Pengaruh fermentasi pada pembuatan MOCAF (Modified Casava Flour) dengan menggunakan ragi roti (Saccharomyces cereviseae), ragi tempe (Rhizopus oryzae), dan Lactobacillus plantarum terhadap kandungan zat nutrisi dan anti nutrisi. Jurnal Teknik ITS, 3(2).

Tuhenay (2018). Pengaruh lama perebusan terhadap kandungan zat besi daun singkong varietas mangi (Manihot esculenta Crantz). Jurnal Mitra Pendidikan, 2(1), 11–22.

Widhiastiti, N.P.U. Darmayanti, LP.T., & Pratiwi, I.D.P.K. (2022). Pengaruh lama fermentasi dengan Lactobacillus plantarum terhadap karakteristik fisikokimia dan fungsional tepung biji durian (Durio zibethinus Murr). Jurnal Ilmu dan Teknologi Pangan (ITEPA), 11(1), 100. https://doi.org/10.24843/itepa.2022.v11.i01.p11

Widyasaputra, R. & Setyo Yuwono, S. (2013). The Effect of Chips Traditional Fermentation To Physical Characteristic of Sweet Potato (Ipomoea batatas L ) Fermented Flour. Jurnal Pangan dan Agroindustri, 1(1), 78–89.

Winarno, F.G. (2004). Kimia Pangan dan Gizi. Jakarta.Gramedia Pustaka Utama.

Wukirsari, T. Saepuddin, E., & Hanafiah., I.V. (2022). Sifat fisikokimia pati tahan cerna hasil hidrolisis asam dan heat moisture treatment pada pati maizena. Jurnal Agritechno, 15(1), 37–43. https://doi.org/10.20956/at.v15i1.587

Wulandari, F., Nazaruddin., & Amaro, M. (2021). Pengaruh jenis bakteria asam laktat dan lama fermentasi terhadap mutu fisikia, kimia, organoleptik dan mikrobiologi tepung MOCAF. Prossiding SAINTEK, 3, 169–181.

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2024-01-29

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