Pathogenicity Efficacy of Entomopathogen Fungus Beauveria bassiana Against In Vitro Rice Stem Borer (Scirpophaga innotata)

Authors

  • Syahbudin Hasibuan Medan Area University
  • Zulfahmi Simbolon Medan Area University
  • Ifan Aulia Candra Medan Area University

DOI:

https://doi.org/10.23960/jtep-l.v13i2.441-448

Abstract

White rice stem borer (Scirpophaga innotata) is one of the dangerous pests for rice (Oryzae sativa) because it attacks rice stems that can cause 50% loss of total production. The purpose of this study was to determine the pathogenicity of Entomopathogen Fungus Beauveria bassiana at different conidia dilutions (J), namely J1 (105), J2 (106), J3 (107), J4 (108), and J5 (109). The mortality of S. innotata larvae was tested with the density of B. bassiana of 2.08 106 conidia/ml. The effect of B. bassiana on the success transformation of S. innotata larvae to pupae for 14 observation day was also observed at the same B. bassiana density. This research resulted in the optimal pathogenicity of B. bassiana at dilution of 107 (treatment J3) which caused 100% death and 100% successful pupation. The lowest treatment was J5 (109 dilution) with a mortality rate of 50% and a successful pupation of 20%. The results of the probit analysis showed that the fastest average lethal time (LT50) value was found in treatment of 107 (J3) which reached 11.11 h.

 

Keywords: Beauveria bassiana, Pathogenicity, Probit analysis, Scirpophaga innotata.

Author Biographies

  • Syahbudin Hasibuan, Medan Area University
    Agrotechnology Departement
  • Zulfahmi Simbolon, Medan Area University
    Agrotechnology Departement
  • Ifan Aulia Candra, Medan Area University
    Departement of Agrotechnology

References

Alfian, R., Solichah, C., & Rizal, A. (2022). Uji kualitas dan patogenisitas jamur Metarhizium anisopliae dan Beauveria bassiana berbagai konsentrasi terhadap hama ulat krop (Crocidolomia pavonana) pada kubis. Agrivet, 28(2), 110-120. https://doi.org/10.31315/agrivet.v28i2.8357

Baron, N.C., Rigobelo, E.C., & Zied, D.C. (2019). Filamentous fungi in biological control: Current status and future perspectives. Chilean Journal of Agricultural Research, 79(2), 307-315. http://dx.doi.org/10.4067/S0718-58392019000200307

Bayu, M.S.Y.I., Prayogo, Y., & Indiati, S.W. (2020). Beauveria bassiana : Biopestisida ramah lingkungan dan efektif untuk mengendalikan hama dan penyakit tanaman. Buletin Palawija, 19(1), 41-63.

Budi, A.S., Afandhi, A., & Puspitarini, R.D. (2013). Patogenisitas jamur entomopatogen Beauveria bassiana Balsamo (Deuteromycetes: Moniliales) pada larva Spodoptera litura Fabricus (Lepidoptera: Noctuidae). Jurnal HPT (Hama Penyakit Tumbuhan), 1(1), 57-65.

Dannon, H.F., Dannon, A.E., Douro-Kpindou, O.K., Zinsou, A.V., Houndete, A.T., Toffa-Mehinto, J., Maurille-Elegbede, I.A.T., Olou, B.D., & Tamò, M. (2020). Toward the efficient use of Beauveria bassiana in integrated cotton insect pest management. Journal of Cotton Research, 3, 1-21. http://dx.doi.org/10.1186/s42397-020-00061-5

Dewata, I., & Danhas, Y.H. (2023). Toksikologi Lingkungan. PT. RajaGrafindo Persada-Rajawali Pers.

Fadhillah, M.A., Agustina, N.A., & Irni, J. (2019). Pengaruh variasi kerapatan spora Beauveria bassiana dan konsentrasi LCPKS terhadap mortalitas larva Oryctes rhinoceros. Agro Estate: Jurnal Budidaya Perkebunan Kelapa Sawit dan Karet, 3(2), 63-72.

Gebremariam, A., Chekol, Y., & Assefa, F. (2022). Extracellular enzyme activity of entomopathogenic fungi, Beauveria bassiana and Metarhizium anisopliae and their pathogenicity potential as a bio-control agent against whitefly pests, Bemisia tabaci and Trialeurodes vaporariorum (Hemiptera: Aleyrodidae). BMC Research Notes, 15(1), 117. https://doi.org/10.1186/s13104-022-06004-4

Halwiyah, N., Fe, S.R., Raharjo, B., & Purwantisari, S. (2019). Uji antagonisme jamur patogen Fusarium solani penyebab penyakit layu pada tanaman cabai dengan menggunakan Beauveria bassiana secara in vitro. Jurnal Akademika Biologi, 8(2), 8-17.

Indriyanti, D.R., Masitoh, M., & Priyono, B. (2016). Keefektifan Metharhizium anisopliae yang dibiakkan di media beras dan yang disimpan di media kaolin terhadap mortalitas larva Oryctes rhinoceros. Life Science: Journal of Biology, 5(1), 64-71.

Jelenić, J., Ćosić, J., Velki, M., & Ilić, J. (2021). Endophytic Fusarium spp. affect yield, but can they be a solution for Botrytis control in vineyard production? Fungal Ecology, 54, 101114. https://doi.org/10.1016/j.funeco.2021.101114

Kahar, S.R.S., Hasan, A., & Lamangantjo, C. (2019). Aktivitas Entomopatogen Serratia marcescens Bizio terhadap mortalitas larva kumbang kelapa (Brontispalongissima) Gestro. JamburaEdu Biosfer Journal, 1(2), 64-71. http://dx.doi.org/10.34312/jebj.v1i2.2430

Kastilong, E.B., Lengkong, M., & Engka, R. (2021). Uji patogenisitas jamur entomopatogen Beauveria bassiana Bals. terhadap walang sangit Leptocorisa acuta Thunb. pada tanaman padi. Cocos, 14(3), 9 pp.

Kirana, V.N., Atmodjo, P.K., & S, B.B.R. (2014). Hubungan variasi perbandingan konsentrasi molase : amonium nitrat terhadap pertumbuhan dan kandungan minyak Aspergillus terreus dan Penicillium pinophilium. https://api.core.ac.uk/oai/oai:e-journal.uajy.ac.id:5387.

Long, W., Chen, Y., Wei, Y., Feng, J., Zhou, D., Cai, B., Qi, D., Zhang, M., Zhao, Y., Li, K., Liu, Y-Z., Wang, W., & Xie, J. (2023). A newly isolated Trichoderma parareesei N4-3 exhibiting a biocontrol potential for banana fusarium wilt by Hyperparasitism. Frontiers in Plant Science, 14, 1289959. https://doi.org/10.3389/fpls.2023.1289959

Mahankuda, B., & Bhatt, B. (2019). Potentialities entomopathogenic fungus Beauveria bassiana as a biocontrol agent: A review. Journal of Entomology and Zoology Studies, 7(5), 870-874.

Ni’mah, Y.K., Afandhi, A., & Choliq, F.A. (2021). Persistensi jamur patogen serangga Beauveria bassiana (Balsamo) Vuillemin (Hypocreales: Cordycipitaceae) pada filoplan tanaman sawi (Brassica rapa L.). Jurnal HPT (Hama dan Penyakit Tumbuhan), 9(2), 57-64). http://dx.doi.org/10.21776/ub.jurnalhpt.2021.009.2.4

Uguy, O.J.R., Montong, V., & Kaligis, J. (2021). Serangan hama penggerek batang padi kuning (Scirpophaga incertulas Wlk.) pada tanaman padi sawah (Oryza Sativa L.) di Desa Liwutung II Kecamatan Pasan Kabupaten Minahasa Tenggara. Cocos, 12(4), 10 pp.

Pant, H., & Verma, J. (2018). Bio-management of root-knot nematode (Meloidogyne incognita) in tomato (Lycopersicon esculeutum L.). ESSENCE Int. J. Env. Rehab. Conserv. IX(2), 60-63. http://dx.doi.org/10.31786/09756272.18.9.2.209

Pelawi, S.P.Br. (2021). Isolasi Cendawan Endofit Pelarut Fosfat dari Akar dan Rizosfer Tanaman Temulawak (Curcuma xantorrhiza Roxb.). [Undergraduate Thesis]. Faculty of Math and Natural Science, Universitas Negeri Padang.

Rahmawasiah, R., Abadi, A.L., Mudjiono, G., & Rizali, A. (2022). The effect of integrated pest management on Scirpophaga innotata population and natural enemies on rice field in South Sulawesi, Indonesia. Biodiversitas Journal of Biological Diversity, 23(9), 4510-4516. http://dx.doi.org/10.13057/biodiv/d230917

Silva-Santana, M.F., Alves, L.F.A., Ferreira, T.T., & Bonini, A.K. (2022). Selection and characterisation of Beauveria bassiana fungus and their potential to control the brown stink bug. Biocontrol Science and Technology, 32(1), 90-102. https://doi.org/10.1080/09583157.2021.1970716

Susanto, M.S., & Prijono, D. (2015). Sinergisme ekstrak Piper aduncum dan Tephrosia vogelii terhadap penggerek batang padi kuning, Scirpophaga incertulas. Jurnal Agrikultura, 26(1), 7-14. http://dx.doi.org/10.24198/agrikultura.v26i1.8454

Telaumbanua, M., Ristanti, R., Amien, E.R., Haryanto, A., & Rahmawati, W. (2020). Teknik pengendalian serangga hama walang sangit (Leptocorisa oratorius) melalui penyemprotan larutan Beauveria bassiana untuk tanaman padi. Jurnal Teknik Pertanian Lampung, 9(4), 374-382. http://dx.doi.org/10.23960/jtep-l.v9i4.374-382

Downloads

Published

2024-05-11

Issue

Vol. 13 No. 2 (2024): June 2024

Section

Articles

License

    1. Authors who publish with this journal agree to the following terms:

        1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution-ShareAlike 4.0 International Lice that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
        2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
        3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).

        4. Jurnal Teknik Pertanian Lampung

          Creative Commons License
          JTEPL is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.